Banca de DEFESA: LARA CRISTINA CARLOS DE MORAIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LARA CRISTINA CARLOS DE MORAIS
DATA : 24/08/2023
HORA: 14:00
LOCAL: Faculdade de Ciências da Saúde - FACS
TÍTULO:

TROPHIC ANALYSIS OF CORTICAL NEURONS WITH ALZHEIMER INDUCTION IN THE PRESENCE OF PANCREATIC CONDITIONED MEDIUM WITH ADDITION OF SUNFLOWER OIL. AN IN VITRO STUDY

PALAVRAS-CHAVES:

Pancreas, Alzheimer's Disease, Sunflower Oil, Amyloid Beta.

PÁGINAS: 81
GRANDE ÁREA: Ciências da Saúde
ÁREA: Saúde Coletiva
RESUMO:

OBJECTIVE: The aim of this study was to analyze the effects of sunflower oil
supplementation with pancreatic cell conditioned medium on Alzheimer-induced cortical
neurons. MATERIAL AND METHOD: Nerve cortical cells were collected from newborn
Wistar rats, the acquisition of the pancreas was from an adult Wistar rat and the beta-amyloid
peptide, which has the ability to induce alzheimer's, was diluted in double distilled water,
having then been cultivated in different groups, a control (D-10) and the others with different
groups (OG, βA, MCP) with cellular morphology evaluated at the end of 96 hours. Cell
expansion data (area, perimeter and axonal length) at 96 hours were statistically compared
using the Kruskal-Wallis test in multiple pairwise comparisons, being considered significant
when p<0.05. RESULT: At the end of 96 hours it was verified that the culture of cells when
cultivated in G7 (D-10, βA, OG) and G8 (MCP, βA, OG) presented morphometric alterations
when compared to the other groups (G2,G3,G4,G5,G6) without joint treatment of MCP and
OG, thus allowing a more evident identification of the morphology of glial and neuronal cells,
resulting in the plastic effect. CONCLUSIONS: Experimental groups treated with GO alone
and in combination with MCP, in the midst of βA showed more pronounced changes when
compared to the control group (D-10), mainly in G7 and G8. Thus, it is shown that OG and
MCP act in neuroprotection, neuroregeneration, acting in the reduction of aggregated
beta-amyloid senile plaques, important for possible treatments of neurodegenerative disorders
such as AD.

MEMBROS DA BANCA:
Interno - 5392 - FAUSTO PIERDONA GUZEN
Externo à Instituição - GEOVAN FIGUERÊDO DE SÁ-FILHO - UFERSA
Interno - 066.871.464-67 - PAULO LEONARDO ARAÚJO DE GÓIS MORAIS - UERN