THE INFLUENCE OF PHYSICAL TRAINING ALONE OR ASSOCIATED TO A HYPER-LIPID DIET OF CASHEW NUTS OR PORK LARGER ON THE OXIDATIVE STRESS OF ADIPOSE TISSUE IN RATS
Adipose tissue, oxidative stress, antioxidant capacity, physical exercise, high-fat diet
PURPOSE: to analyze the effects of aerobic training alone or associated with a high-fat diet based on pork lard and cashew nuts on oxidative stress in the adipose tissues of female rats. METHODOLOGY: The sample consisted of 96 female Wistar rats randomized into groups called control (CON), cashew nut (CC) and lard (BP), 48 of these were sedentary who only administered a high-fat diet and 48 animals associated diet with physical training on a treadmill. The groups were also divided into an intervention period of 3 and 6 months. All training and euthanasia protocols were performed taking ethical care for animal research. Samples were extracted and packaged until analysis. Brown adipose tissues, puri-uterine, mesentery, abdominal cavity. Analyzes of Malondialdehyde (MDA) as an oxidizing agent and Total Antioxidant Capacity (CAOT) were carried out using the method based on the elimination of the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). Anova one way test with Bofferroni's post-hoc was used to verify possible differences, considering the factors group (diet CC, BP CON), exercise (isolated exercise, exercise + CC and exercise + BP) and time (three months and six months); independent t test to verify possible differences between the initial values of weight, MDA and CAOT between groups and between tissues; Pearson's correlation was used to verify possible relationship between weight, e MDA and CAOT; The significance level adopted was p<0.05. RESULTS: The MDA of the sedentary and exercise group, both the 3 and 6 months intervention of the cashew nut groups showed a lower concentration of lipid peroxidation, respectively in the aforementioned groups and periods (CC: 0.25±0.012μM; 0.24 ±0.009μM. EXCC: 0.45±0.24μM; 0.32±0.14μM) in all tissues studied and when compared to the other groups studied. The null hypotheses were all refuted, with the alternative hypotheses prevailing. In the 3-month intervention in sedentary and exercise groups related to cashew nuts showed an increase in CAOT (CC: 35.45±7.56%; EXCC: 36.46±8.38%). In brown adipose tissue, puri-uterine and abdominal cavity. As for the intervention in the exercises, the cashew brown groups presented a CAOT (40.02±9.2%) greater than that in the brown adipose tissues. CONCLUSION: A diet based on cashew nuts, associated or not with exercise, showed a decrease in lipid peroxidation and a greater total antioxidant capacity in adipose tissue in female rats with three and six months of intervention.