Banca de DEFESA: MARCÍLIA INGRID LIMA BARROSO NUNES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARCÍLIA INGRID LIMA BARROSO NUNES
DATA : 29/08/2023
HORA: 08:00
LOCAL: Google Meeting
TÍTULO:

EFFECTS OF DIFFERENT SOURCES OF FAT IN THE HYPER-LIPID DIET ASSOCIATED WITH PHYSICAL TRAINING ON OXIDATIVE STRESS IN RAT HIPPOCAMPUS

PALAVRAS-CHAVES:

Hippocampus; exercise; diet high-fat; oxidative stress; anti-oxidant effect.

PÁGINAS: 48
GRANDE ÁREA: Ciências da Saúde
ÁREA: Educação Física
RESUMO:

Introduction: The composition and control of the components of a diet are directly related to
the physiological mechanisms in the modulation of the function of the molecular systems
involved in the maintenance of health and, above all, in the neuronal activity. The hippocampus,
commonly related to cognitive processes such as memory and learning, seems to be particularly
susceptible to morphological and functional changes resulting from the consumption of a highfat
diet. Physical training may be a useful therapeutic tool with the aim of combating the
neurodegenerative pathophysiology arising from high-fat diet consumption and the
mechanisms underlying the low-fat diet-induced decreases in learning and memory function.
Objective: to analyze the effects of different types of fat sources in the high-fat diet, whether
animal or vegetable, associated with physical training on oxidative stress in the hippocampus
of rats. Methodology: Wistar rats (n=48) were used, with food and water provided ad libitum,
submitted to physical training for 6 months. The animals were divided into 6 groups according
to diet and training: group 1- standard ration and sedentary; group 2- hyperlipidic diet with lard
and sedentary; group 3- hyperlipidic diet with cashew nuts and sedentary; group 4 – standard
ration and exercise; group 5 – high fat diet with lard and exercise and group 6 – high fat diet
with cashew nuts and exercise. Euthanasia took place during the rats’ estrous cycle. After
euthanasia of the rats, the brain was removed from the braincase to separate the hippocampus
areas to evaluate the hippocampal levels of MDA, SOD and free protein Values were expressed
as mean ± standard deviation. Two-way ANOVA with repeated measures was used and the
Bofferroni’s post hoc was defined as a function of the coefficient of variation. The significance
level adopted will be α ≤ 0.05 with variables have normal and homoscedastic distribution.
Results: It was verified that the high-fat diet based on pork lard caused an increase in MDA in
the hippocampus of female rats when compared with the diet based on cashew nuts and control
(BHP: 0.18±0.06 % vs. CNT : 0.14±0.03%; p=0.04; BHP: 0.18±0.06% vs. CSC: 0.12±0.03%;
p=0.00), without significant changes in protein levels free. Physical exercise was only able to
reduce the levels of free proteins in the hippocampus in the group of rats on a control diet
(SDCNT: 1.00±0.23 μl vs. EXCNT: 0.73±0.08 μl; p=0.024), without changes in hippocampal
levels of MDA. As for SOD, there was an increase in the sedentary group with lard diet in
relation to the other sedentary groups (SDBHP: 12.64±11.05% vs. SDCNT: 3.40±1.91%; p=
0.00; (SDBHP: 12.64±11.05% vs. SDCSC: 6.04±3.83%; p=0.05). Finally, there was weight
gain in all groups at the end of the experiment, with no significant difference in weight between
the groups. Conclusion: After 6 months of physical training, the reduction in free protein levels
in the hippocampus was only observed in the groups with control food, without alterations when
other diets were used. The levels of MDA only changed in the groups with a lard-based diet,
regardless of whether or not they practiced physical exercise and hippocampal levels of SOD
only showed an increase in the sedentary group using lard. In addition, diet and the practice or
not of physical exercise had no impact on the weight gain of the sample.

MEMBROS DA BANCA:
Externo à Instituição - FRANCISCO SÉRGIO LOPES VASCONCELOS FILHO - UFCA
Externo ao Programa - 4525 - ADALBERTO VERONESE DA COSTA
Interna - 12250 - IVANA ALICE TEIXEIRA FONSECA