Banca de DEFESA: ANDRE LUIZ DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANDRE LUIZ DA SILVA
DATA : 31/03/2022
HORA: 14:00
LOCAL: https://meet.google.com/sgp-dioo-eff
TÍTULO:

ANTIFUNGAL ACTIVITY OF PROTEINS EXTRACTED FROM Luffa operculata SEEDS AGAINST Candida albicans and Candida krusei.

PALAVRAS-CHAVES:

Antifungal proteins; Biotechnological potential; Plant extracts.

PÁGINAS: 47
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

 Luffa operculata is a vine species native to Brazil widely used for the treatment of respiratory diseases, howerver, studies indicate its effectiveness in the treatment of other diseases. Candida spp. are fungi that annually kill approximately 1.5 million people. Within this genus, C. albicans e C. krusei species are human pathogens that cause a high death rate of infected. Treatments for diseases caused by these microorganisms consist of antifungals, which can cause fungal resistance. The interest in plant species that have biotechnological applications is of great interest to society. Thus, this research aimed to evaluate the antifungal potential of protein extracts from Luffa operculata seeds against C. albicans e C. krusei fungi. In this perspective, seeds of the species were crushed to form the protein flour that originated the extracts. From these extracts, the parameters were evaluated: electrophoretic profile of the proteins extracted from the seeds; concentration of soluble proteins; growth inhibition rate of these microorganisms; enzymatic activities; pore formation through fluorescence assays and morphological aspects of the fungal cell through scanning electron microscopy. Test samples were used in triplicate. The data obtained were submitted to ANOVA, followed by Tukey's test, using the GraphPad Prism, with a significance of p ≤ 0.05. The results showed that proteins from the seeds of L. operculata have antifungal activity against C. albicans and C. krusei fungi. Which is observed when analyzing the rate of inhibition of fungal growth for the species, observing the scanning electron microscopy images that demonstrate damage to the surface of fungal cells, and fluorescence microscopy that show the formation of pores in the membrane and cell wall of fungi, as well as the formation of reactive oxygen species, indicating the extracts from seeds of this species as a potential plant resource for the treatment of diseases caused by C. albicans and C. krusei.

MEMBROS DA BANCA:
Externo à Instituição - LUIZ PALHARES NETO
Externo à Instituição - PEDRO FILHO NORONHA DE SOUZA
Presidente - 3396 - CYNTHIA CAVALCANTI DE ALBUQUERQUE
Externa ao Programa - 1635 - FRANCISCA MARTA MACHADO CASADO DE ARAÚJO