Banca de DEFESA: JOYCE SOARES DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOYCE SOARES DE SOUZA
DATA : 30/08/2023
HORA: 08:00
LOCAL: Videoconferência
TÍTULO:

Synthesis of eugenyl acetate catalyzed by zeolites and evaluation of its anxiolytic potential.

PALAVRAS-CHAVES:

Zeolites, Eugenol, Esterification, Heterogeneous catalysis.

PÁGINAS: 73
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

Eugenyl acetate (EA) is a derivative of eugenol and has antifungal, antimicrobial, larvicidal and other actions. One of the ways to obtain this compound is through the esterification reaction, and for the development of a chemical process that is less harmful to the environment, heterogeneous catalysis is an alternative, since chemical synthesis with these catalysts has shown promise. Among these catalysts, zeolites have been studied in esterification reactions, with promising results. Therefore, this work aimed to synthesize ZSM-5 and Beta zeolites and use them in the eugenol esterification reaction, in order to evaluate the ester obtained for anxiolytic activity. The catalysts were synthesized and characterized by X-ray diffraction, chemical analysis and textural analysis by N2 adsorption. The esterification reactions were carried out with excess and limiting eugenol, acetic anhydride and 3% and 2% catalyst (ZSM-5 and Beta with and without ion exchange), in order to evaluate the reaction time between the catalysts. Through the technique of thin layer chromatography (TLC) and Ultra Performance Liquid Chromatography Mass Spectrometry (HPLC-MS) it was possible to monitor the reactions and the formation of the AE as a function of time. The kinetic study demonstrated that the conversion in a minimum time of 60 minutes in the limiting eugenol reaction was achieved with Beta zeolite, followed by ZSM-5 with 120 minutes of reaction. Catalyst reuse was studied with Beta, which presented 3 reuse cycles, with an increase of 30 minutes in each cycle. The anxiolytic test was performed in vivo in Zebrafish, locomotor activity was evaluated (open field test - OFT), Acute toxicity 96 h and the anxiolytic effect was evaluated in the light/dark test. The AE did not show toxicity to adult zebrafish up to 96 h of analysis, it was observed that the highest dose of AE (40 mg/kg) caused motor impairment, unlike the lower concentrations of 4 and 20 mg/kg in the sample, which did not cause impairment. The AE proved to be anxiolytic, as the treated animals remained most of the analysis time in the clear zone of the aquarium. In this way, the methods used in the present work are efficient for obtaining the catalysts and eugenyl acetate, since its synthesis with Beta and ZSM-5 zeolites and without solvents makes the process less harmful to the environment, in addition to the anxiolytic activity confirmed for eugenyl acetate, showing potential to be used in the development of compounds with anxiolytic properties.

MEMBROS DA BANCA:
Presidente - 12281 - VINICIUS PATRICIO SANTOS CALDEIRA
Interno - 12768 - KEURISON FIGUEREDO MAGALHÃES
Externo à Instituição - HÉLCIO SILVA DOS SANTOS - UEVA
Externo à Instituição - WILDSON MAX BARBOSA DA SILVA - UECE